Abstract
Prevalence of Virulance Gene by Real Time Pcr of Legionella pneumophila Strains Isolated from Different Sources
Highlights
Legionella is an intracellular pathogen that parasites host mononuclear phagocytes
A total of 25 L. pneumophila isolate (10 clinical and 15 environmental ), 1 standard L. pneumophila serogroup 1 (ATCC 43111), 1 Legionella pneumophila serogroup 1 standard strain(ATCC 33152), Legionella bozemanii(ATCC 33217) and Legionella micdadei(ATCC 33218) standard strain was tested in this study
All samples were examined by Real Time PCR for type II secretion system, type IV secretion system and quarum sensing cluster using Mip, RtxA, Legionella quorum sensing cluster (Lqs) and HdeD gene region
Summary
There have nearly 59 species and 70 distinct antigenic types of Legionella. Of the 50 species, 20 are disease-causing agents [1,2]. Between the these species; Legionella pneumophila is an ethiological agent of Legionnaires’ disease and the most common species detected in clinical cases (80-90%), other species have been reported to cause disease. It consists of 16 serogroups [3,4]. Other Legionella species have been reported to cause disease. The most common species after L. pneumophila is L. micdadei [5]
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