[Homology of Legionella strains isolated from clinical cases and environmental water in Shenyang].

Abstract

Objective: To study the homology of Legionella pneumophila (LP) strains which were isolated from clinical specimens and environmental water. Methods: Twenty-two Legionella strains were collected in which 2 strains were isolated from 2 cases of LP infection patients, and others were isolated from domestic water or air conditioning cooling tower water of 2 hospitals and 13 companies from July 2012 to September 2014 in Shenyang. They were recovered and analyzed by the pulse field gel electrophoresis (PFGE) with AscⅠ restriction enzymes. Data were cluster analyzed using SPSS 19.0. Results: Twenty of 22 strains were LP1, and others were LP7 and LP8. Twenty-two strains could be divided into 14 genotypes, and 7 strains belonged to SYC banding pattern. The same bacterial serotype presented with different genotypes. Different bacterial serotypes and genotypes of LP strains can be found in the same place, and homology could be observed between LP strains isolated from cooling tower water and domestic water within the same hospital. Conclusion: In Shenyang area, LP strains which are isolated from clinical cases and environmental water have the characteristics of gene polymorphism. Legionella strains isolated from different water system in the same hospital have homology.