Abstract
ABSTRACT A collection of 500 Aspergillus flavus isolates from four sesame varieties (S-34, S-35, S-38, and S-39) that were planted in field plots in the Mississippi Delta and in the Florida Panhandle were investigated because of low-level aflatoxin contamination detected in sesame seeds. A rapid molecular fingerprinting method was developed to assess the influence of prior applications of the atoxigenic Afla-Guard® biocontrol product whose active strain is NRRL21882 on the A. flavus populations within each field plot. Depending on sesame seed sampled, 66.7% to 95.9% of A. flavus isolates from Mississippi belonged to the NRRL21882 genotype, which lacks the aflatoxin and cyclopiazonic acid biosynthesis gene clusters. In contrast, only 5.0% to 32.5% of the isolates from Florida had lost both gene clusters. The high incidence of NRRL21882-like A. flavus in Mississippi sesame samples can be attributed to prior applications of Afla-Guard® in that local area. The results suggest the adaptability of this particular type of atoxigenic A. flavus biocontrol strain in the field.
Published Version
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