Abstract
Since feline coronavirus (FCoVs) are ubiquitous pathogens, the seroprevalence was studied to monitor infection in Turkey. One hundred sera, collected from cats belonging to catteries or community shelters and to households, were tested. The VN test was performed and compared with ELISA. Each sample was heat-inactivated and serial two-fold dilutions starting from 1/2 were mixed with 100TCID50/50μl of FCoV type II strain 25/92. Subsequently, freshly trypsinized CRFK cells were added and the plates were incubated at 37°C for 96h.
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