Abstract
Bovine Herpes Virus (BHV-1) is a virus prevalent among cattle and buffaloes which accounts for considerable reproductive failures. This study was undertaken with the objective of studying the prevalence of BHV-1 in reproductive tract infections of cattle and buffaloes in Punjab region in India. A total of 70 reproductive tract samples (like vaginal mucous, cervical mucous, uterine discharges, uterine pus and aborted materials like placenta, caruncles, foetal stomach contents, amniotic fluid and placental fluid) were taken from cattle and buffaloes from various areas of Punjab which were suffering from different reproductive disorders. The samples were screened for the presence of genome of BHV-1using PCR targeting gE gene. Out of 70 samples screened, only one sample was positive for the presence of BHV-1 genome which had an amplicon size of 468bp, specific to the targeted gene. The study concluded that BHV-1 has very low prevalence among reproductive disorders in cattle and buffaloes in Punjab region, but has increased over last few years, particularly in female cattle and buffaloes.
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