Prevalence, Molecular Characterization and Antimicrobial Resistance of Vero Toxigenic E.Coli in Fresh Soft Cheese, Ice Cream and Yoghurt in Mansoura City

Abstract

A total of 130 samples of fresh soft cheese (Talaga) (30), ice cream (50) and yoghurt (50) were taken randomly from markets besides small sellers in various regions of Mansoura town, Egypt then examined for the prevalence of cytotoxigenic Escherichia coli. The suspected strains were serological typed then examined for the existence of Vero toxigenic Escherichia coli (VTEC) via Multiplex Polymerase Chain Reaction. The exposure to fourteen antibiotics disks was assessed by the disk dispersal way. Results obtained showed that toxigenic E.coli was discovered in 3% (4/130) of the total examined samples with incidence of 10% (3/30), 2% (1/50) and 0% (0/50) in fresh soft cheese, small scale ice cream, traditional yoghurt, respectively. The serogroups identified were one O26: H11, Three O55: H7 and one O91: H21. E. coli O157 was not found in all examined samples. Maximum repeated durability was detected to the next antibiotics: Streptomycin (100.0%), Nalidixic acid (80.0%), Erythromycin (80.0%), Amikacin (60.0%) and Cefotaxim (60.0%). Sulphamethoxazol (60.0%), Tetracycline (60.0%), Cephalothin (40.0%), Chloramphenicol (40.0%), Ampicillin (40.0%), Kanamycin (40.0%), Ciprofloxacin (40.0%), Neomycin (20.0%) and Gentamicin (20.0%). Conclusively, some of fresh soft cheese (Talaga) and small scale production ice cream at retail markets located in Mansoura city haven multidrug-resistant VTEC.