Abstract
Haemophilus parasuis, the causative agent of Glässer’s disease, has been reported as widespread, but little is known about its epidemiology in the Sichuan province of China. The goal of our research is to reveal the prevalence and distribution of H. parasuis in this area. Sampling and isolation were performed across Sichuan; isolates were processed using serotyping multiplex PCR (serotyping-mPCR) and agar gel diffusion (AGD) for confirmation of serovar identity. This study was carried out from January 2014 to May 2016 and 254 H. parasuis field strains were isolated from 576 clinical samples collected from pigs displaying clinical symptoms. The isolation frequency was 44.10%. Statistically very significant differences of infection incidence were found in three age groups (P < 0.01) and different seasons (P < 0.01). Serovars 5 (25.98%) and 4 (23.62%) were the most prevalent, however, non-typeable isolates accounted for nearly 7.87%. In terms of geographical distribution, serovars 5 and 4 were mostly prevalent in west and east Sichuan. The results confirmed that the combined approach was dependable and revealed the diversity and distribution of serovars in Sichuan province, which is vital for efforts aimed at developing vaccine candidates allowing for the prevention or control of H. parasuis outbreaks.
Highlights
Haemophilus parasuis, a member of the family Pasteurellaceae, colonises in the upper respiratory tract of healthy pigs during different breeding periods, and emerges as the aetiological agent of Glässer’s disease in some cases (Oliveira & Pijoan, 2004)
As no detailed report of H. parasuis infection was available in Sichuan province, China, we investigated the prevalence and seroepidemiology of H. parasuis in Sichuan province from January 2014 to May 2016
A total of 254 field H. parasuis isolates were confirmed via viewing culture morphology characteristics and 16S rRNA PCR results, with the isolation rate of 44.10%
Summary
Haemophilus parasuis, a member of the family Pasteurellaceae, colonises in the upper respiratory tract of healthy pigs during different breeding periods, and emerges as the aetiological agent of Glässer’s disease in some cases (Oliveira & Pijoan, 2004). This organism incurs significant economic losses within the swine industry. Various molecular approaches have revealed the diversity in genotypic lineage and have shed light on the heterogeneity of pathogenic H. parasuis serovars (Olvera, Calsamiglia & Aragon, 2006; Del Río et al, 2006; Zhang et al, 2011).
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