Abstract
Prevalence and Distribution of African Swine Fever Virus in Swine Feed After Mixing and Feed Batch Sequencing
Highlights
The porcine epidemic diarrhea virus (PEDV) outbreak of 2013–2014 was the first major disease outbreak to suggest a potential link between contaminated feed and pathogen transmission in pigs.[6]
The objectives of this study were to 1) evaluate feed batch sequencing as a mitigation technique for ASFV contamination in a feed mill, and 2) determine if a feed sampling method could identify ASFV following experimental inoculation
Quantity of ASFV p72 DNA decreased sequentially as additional batches of initially ASFV-free feed were manufactured, but it was still detectable after batch sequence 4, suggesting cross contamination between batches
Summary
The porcine epidemic diarrhea virus (PEDV) outbreak of 2013–2014 was the first major disease outbreak to suggest a potential link between contaminated feed and pathogen transmission in pigs.[6] This hypothesis was never unequivocally proven. It has been suggested that mitigation measures common in PEDV, such as feed batch sequencing to reduce viral concentration, may be effective with ASFV. Both of these strategies have never been evaluated. The objectives of this study were to 1) determine if a common sampling strategy could consistently detect ASFV in feed, and 2) evaluate if feed batch sequencing could serve as a potential mitigation technique for ASFV contamination during feed manufacturing
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