Abstract
A rapid and efficient process for the separation of chitin from waste prawn shells using hot glycerol pretreatment is reported. The pretreatment of waste prawn shell in hot glycerol enables the removal of protein possibly through dehydration and temperature induced fragmentation into low molecular weight water-soluble fragments, which are subsequently removed from the shell matrix by dissolution in water. In contrast, in the industrial process of preparing chitin from crustacean shells, the deproteinization is carried out with hot aqueous sodium hydroxide. The novel pretreatment present here should be applicable to all crustacean shell waste, in principle. Chitin was isolated by two different methods after the pretreatment in glycerol. In one of the methods, the pretreated shells were treated directly with citric acid to remove protein and minerals (mostly as calcium citrate). In the second method, the pretreated shells were ground and rinsed with water to remove protein fragments and part of the minerals...
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