Abstract
Prawn shell waste (PSW) from seafood and aquaculture processing industries is currently a serious environmental concern due to improper disposal practices. Current methods for production of value added products like chitin, chitosan, pigments and protein from PSW uses harsh chemicals leading to generation of polluting effluents and green alternatives are highly desirable. Bacillus megatarium and B. subtilis, isolated from PSW were evaluated for their potential in demineralization (DM) and deproteination (DP) the shell waste for production of chitin and protein hydrolysate, alone or in combination. PSW fermentation with B. megatarium gave 73% DM and 73.28% DP when supplemented with 0.1% glucose, whereas, co-fermentation using both cultures gave DM and DP efficiencies of 70.75% and 75.12% respectively. Chitin recovery was maximum (40%) for B. megatarium and FTIR analysis showed characteristic signatures of chitin. Protein hydrolysate was enriched in essential amino-acids indicating potential to be used in feed additives.
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