Abstract
The effect of tumor necrosis factor-α (TNF-α) on the stimulation-evoked release of noradrenaline (NA) from isolated rat median eminence (ME) was investigated, using a low-volume perfusion system. Median eminence, loaded with [ 3H]noradrenaline, was superfused with Krebs solution and stimulated electrically (2 Hz, 120 shocks). The effect of TNF-α was studied on the S 2 S 1 ration. It was found that stimulation-evoked relesed of NA from noradrenergic axon terminals in the isolated rat ME was inhibited by TNF-α and this effect was concentration-dependent. In contrast, TNF-α had no effect on the release of [ 3H]NA from the spleen. Since NA released in the ME might be involved in the modulation of corticotropin-releasing factor (CRF) production, it is suggested that TNF-α, through presynaptic modulation of NA release from noradrenergic nerve terminals in the ME, might regulate CRF and other neurohormone release in this hypothalamic structure.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call