Preservation of Retinoid Influx into Eye Tissues of ABCR-Deficient Mice

Abstract

Using an in vivo radiolabeling technique, we investigated the movement of retinoid into the retinal pigment epithelium (RPE) of the abcr-/- mouse, which lacks the photoreceptor ABCR protein and is a model for Stargardt disease. Eye tissues and serum obtained from dark-adapted, 5- to 8-month-old abcr-/- and control mice following the intraperitoneal injection of all-trans (3H)retinol were analyzed to determine the inferred influx of retinoid from the serum into the RPE. At 4.5 hr post-injection, the inferred all-trans retinol influx in abcr-/- mice, which possess the leucine 450 variant of RPE65 protein, was 0.011 ± 0.004 nmol (n = 3). This value did not differ significantly from that determined in age-matched controls possessing the methionine 450 variant of RPE65 (0.015 ± 0.003 nmol; n = 3) or from 3-month-old wildtype mice that possess the leucine 450 RPE65 variant (0.020 ± 0.007; n = 4). Thus, the absence of ABCR does not significantly compromise the passage of retinoid from the serum into the RPE under dark-adapted conditions.