Abstract
Objective To compare preservation by the standard method of simple cold storage in HTK solution with that by extracorporeal fiver perfusion.Methods Livers were harvested from health pigs,which were preserved for 10 h by either cold storage in HTK solution (group A,n=4) or by perfusion with oxygenated autologous blood at body temperature (group B,n=4).Both groups were then tested on the circuit for a 4 h reperfusion phase,and the data of bile production,hemodynamic parameters and the markers of hepatoeyte and reperfusion injury of extracorporeal livers in each were analyzed at the end of reperfusion.Results At 1st,2nd,3rd and 4th h after reperfusion,the data of bile production,hemodynamic parameters and the markers of hepatocyte and reperfusion injury of livers in group A were statistically different from those of livers in group B (P＜0.05 or P＜0.01).Conclusion Compared with simple cold storage in HTK solution,extracorporeal liver perfusion iS a superior method of preservation to non-heart-beating donor livers. Key words: Liver; Perfusion; Organ preservation solutions; Cryopreservation
Published Version
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