Abstract
Purpose: Degeneration of pericytes and acellular capillaries are increased in the feet of human diabetic patients. Selective loss of adhesion of pericyte to endothelial cell is known to early event in diabetic microangiopathy. In diabetes, inflammation due to endothelial dysfunction is augmented. Cilostazol, a PDE III inhibitor, is reported to reduce inflammation and oxidative stress in diabetic patients. Based on these findings, we investigated the effect of cilostazol on endothelial/pericyte adhesion in ischemic hindlimb of diabetic mice.Method: Type I diabetes was induced by intraperitoneal administration of streptozocin (50mg/kg) for five days in both cilostazol‐fed mice and control‐diet‐fed mice. Resection of femoral artery was conducted to develop hindlimb ischemia. Ten days after the procedure, gastrocnemius muscles were resected. Adhesion of pericyte to endothelial cell was assessed by transmission electron microscopy.Result: The rate of detachment between endothelial cell and pericyte in cilostazol‐fed mice was 1/15, whereas it was 9/8 in non‐cilostazol‐fed mice (p<0.005).Conclusion: Cilostazol preserved endothelial cell/pericyte adhesion in ischemic limb of diabetic mouse. Cilostazol may provide improvement of capillary morphology in diabetic foot in addition to vasodilation.
Published Version
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