Abstract
Summary - The preservation of genomic DNA in spinach leaves by desiccation, using drierite and silica gel, was examined. Drierite and silica gel were found to be equally effective in preserving DNA in spinach leaves for up to 6 months at 37°C. Similar results were obtained using Juniperus leaves but Magnolia leaves had more degradation and oak ( Quercus ) leaves had considerable degradation after 6 months storage in silica gel at 37°C. Storage tests involving fresh and dried spinach leaves indicated that either materials can be used, but air dried spinach leaves had slightly less DNA degradation at each sample interval. The use of either silica gel or drierite appears to be an acceptable method for the preservation of DNA in tropical plant leaf specimens for subsequent extraction and archiving or analyses. A practical field protocol for DNA collection is presented.
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