Preservation and DNA Fingerprinting of the Historic Tidal Basin Cherries

Abstract

Abstract The historic Japanese flowering cherry trees planted around the Tidal Basin in Washington, DC, were given to the United States in 1912 as a gift from Japan, yet only a small portion of the original trees remain. In cooperation with the National Park Service, the U.S. National Arboretum clonally propagated a portion of these trees. DNA from these and other P. x yedoensis plants obtained from domestic commercial nurseries were compared using RAPD markers. Twenty-one 10-nucleotide primers yielded 80 repeatable bands that were used to assess genetic distances among the accessions. The genetic distances ranged from 0.65 to 1.0, with thirteen accessions identical at all loci tested. The most genetically dissimilar trees were P. x yedoensis accessions that were collected as seed in Japan. Accessions obtained from commercial nurseries including ‘Afterglow’, ‘Akebono’, and Yoshino were also dissimilar to the Tidal Basin trees. This study indicated that most of the older trees planted around the Tidal Basin are genetically very similar, but that variability in P. x yedoensis exists, especially in accessions collected as seed from Japan.