Presencia de Ocratoxina A (OTA), de Citrinina y de Fumonisina B1 en cereales para desayuno colectados en Francia. Comparación del análisis de OTA con utilización o no de una purificación con columna de immunoafinidad antes de la cromatografía de líquidos

Abstract

Cereals and other crops are susceptible to fungal attack either in the field or during storage. The mycotoxins produced by these fungi are very stable and could be found in final products such as breakfast cereal. The aim of this study was to evaluate the presence of Fumonisin B (FB ), Ochratoxin A (OTA) and Citrinin (CIT) in some breakfast cereals collected 1 1 in French supermarkets. FB was purified by immunoaffinity columns and has been 1 detected, after derivatization, in 25/27 samples, ranging from 1 to 1110 μg/kg. FB has been 1 detected in samples containing corn, but also oat or rice. OTA and CIT recoveries were over 80 % by the conventional method of partition extraction, whereas the OTA recovery was about 60% using immunoa finity clean-up in phosphate saline buffer. OTA has been detected in 72.5% of the samples ranging from the limit of detection (0.1μg/kg) to 8.8 μg/kg. This highest level was detected in a sample containing dry fruit and fibres. Seventeen percent of the samples contained OTA above the EU limit of 3μg/kg. Twenty percent of them were also contaminated by CIT.