Abstract
The phenotypic characteristics associated with the virulence plasmid in Yersinia enterocolitica are expressed only at 37C, a temperature which facilitates the loss of the plasmid. This report investigated the stability of the virulence plasmid in Y. enterocolitica after its phenotypic expression at 37C. Polymerase chain reaction analysis of plasmid DNA showed that Y. enterocolitica did not loose the virulence plasmid during phenotypic expression as assayed by crystal violet binding, Congo red uptake, low calcium response, hydrophobicity and autoagglutination at 37Cfor 24 h. Prolonged incubation of at least 48 h at 37C is needed to trigger the loss of the plasmid.
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