Abstract
ABSTRACT A novel method was developed and evaluated for detecting Shigella by a loop‐mediated isothermal amplification (LAMP) in this paper. Specific primers were specially designed and selected for recognizing seven distinct sequences of ipaH gene, which was performed only by Shigella. The sensitivity of the LAMP assay was 43 cfu/mL, approximately equal to that of real‐time polymerase chain reaction assay. The LAMP assay developed in this study is specific, sensitive and suitable for the detection of foodborne Shigella.PRACTICAL APPLICATIONThe LAMP method reported here provided a powerful tool for detection of foodborne Shigella due to its specificity, sensitivity and rapidity.
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