Abstract
This article reports data describing HO-1 expression patterns of heme oxygenase (HO)-1 in isolated rat glomeruli and in cultured glomerular epithelial cells (GEC) in response to its natural substrate heme. Qualitative and quantitative data are presented to support presence of a HO-1 expression threshold in glomeruli but not in GEC. Interpretation of our data and further insight into HO-1 expression pattern in glomeruli may be found in ‘HO-1 expression control in the rat glomerulus’ [1].
Highlights
This article reports data describing HO-1 expression patterns of heme oxygenase (HO)-1 in isolated rat glomeruli and in cultured glomerular epithelial cells (GEC) in response to its natural substrate heme
Analysed Glomeruli isolated from kidneys or glomerular epithelial cells (GEC) were incubated with various concentrations of hemin or volumes of hemopexin (HPX) deficient serum for 18 h Pre-treated glomeruli or GEC were processed for western blotting or Real-time PCR amplification Athens, Greece
We previously demonstrated that HO-1 induction in the renal glomerulus in response to its natural substrate/inducer, heme, reaches a threshold beyond which protein synthesis is halted and HO-1 protein levels are markedly reduced
Summary
Analysed Glomeruli isolated from kidneys or glomerular epithelial cells (GEC) were incubated with various concentrations of hemin or volumes of hemopexin (HPX) deficient serum for 18 h Pre-treated glomeruli or GEC were processed for western blotting or Real-time PCR amplification Athens, Greece. These data support presence of an HO-1 expression threshold in the renal glomerulus in response to its natural substrate, heme. The data demonstrate that, in contrast to isolated glomeruli, heme-mediated HO-1 induction threshold is not reached in a cellular component of glomeruli (GEC) (Fig. 3)
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