Prescribed fire affects the concentration and aromaticity of soluble soil organic matter in forest soils

Abstract

Prescribed burning is used widely across Australia to reduce fuel load and associated wildfire hazard. However, prescribed burning can influence carbon (C) storage in affected ecosystems, potentially influencing C cycling. One important component of the C cycle is soluble C, which is available to microorganisms and therefore a key driver of nutrient cycling. However, studies of the effects of fire on soluble C in natural ecosystems are few. In this study, UV–Vis spectra of water extracts of soils from an Australian Eucalyptus forest were used to investigate soluble soil C characteristics to 30 cm depth after autumn burning every three or ten years for three decades contrasted with long non-burnt controls. A random forest prediction model was fit to the UV–VIS spectra and the wavelengths important to predicting soluble C investigated using conditional inference trees. The main absorbance of the UV–Vis spectra was in the aromatic region (~280 nm) indicating chemically complex soluble organic matter in both burnt and non-burnt soils. Water extractable organic carbon decreased significantly with depth and was reduced by 3-yearly burning in surface soils. Furthermore, burning, irrespective of treatment, led to significant shifts in the locations of soil spectra peaks, which were consistent with both lower quantities of non-aromatic substances and less substituted aromatic substances in the burnt than non-burnt sites. These results indicate that repeated prescribed fire over decades reduced the quantity of soluble C, and enhanced its aromaticity, potentially reducing biological availability and therefore nutrient cycling in the soils.