Abstract
Five known chromones were isolated and purified from Saposhnikovia divaricata for the first time by a combination of reverse-phase medium-pressure liquid chromatography (RP-MPLC) and high performance counter-current chromatography (HPCCC). The n-butanol extracts of S. divaricata were preliminarily chromatographed by RP-MPLC and further isolated by preparative HPCCC. A total of 582.8 mg of prim-O-glucosylcimifugin, 228.9 mg of 4′-O-β-D-glucosyl-5-O- methylvisamminol, 113 mg of cimifugin, 14.5 mg of sec-O-glucosylhamaudol, and 4.68 mg of 5-O-methylvisamminol were purified from 7.48 g of the n-butanol extract of S. divaricata, each at over 95% purity as determined by high performance liquid chromatography (HPLC). The identification of the five purified compounds was achieved by congruent retention times, the data of the liquid chromatography- electrospray ionization mass spectrometry (LC-ESI-MSn), and nuclear magnetic resonance spectroscopy (NMR) with those of the authentic standards and literature reports.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
