Preparative separation of hyperoside of seeds extract of Saposhnikovia divaricata by high performance counter-current chromatography

Abstract

Hyperoside was isolated and purified from the seeds of Saposhnikovia divaricata for the first time by high performance counter-current chromatography (HPCCC) using a solvent system of ethyl acetate-n-butanol-ethanol -water (1:1:0.1:2, v/v/v/v). By injecting ca. 500 mg of n-butanol extract of S. divaricata seeds for five times, a total of 232.7 mg of hyperoside was purified from 2.5 g of the n-butanol extract of S. divaricata seeds, at 96.3% purity as determined by high performance liquid chromatography (HPLC). The identification of the purified compound was achieved by congruent retention time, ultraviolet (UV) spectra and the data of high-performance liquid chromatography- electrospray ion source mass spectroscopy (LC-ESI-MSn) in the positive mode with that of the authentic standard and literature reports. Key words: S. divaricata seeds, hyperoside, HPCCC.