Abstract

New preparative high-performance liquid chromatographic techniques that allow the practical isolation of milligram to gram quantities of malto-oligosaccharides from starch hydrolysates are described. High-resolution fractionations were achieved on preparative ( − 30 × 2.0 cm) columns packed with either (i) H +-form cation-exchange resin, (ii) Ag +-form cation-exchange resin, (iii) aminopropyl silica gel, or (iv) octadecyl (C 18) silica gel. Details are given for the preparation and packing of the first three phases and for the practical use of all four for rapid oligosaccharide fractionations. Although each column had some unique features, they all operated at surprisingly low flow-rates (2–12 ml/min) and pressures (15–1000 p.s.i.). In addition, most were relatively easy and inexpensive to assemble and operate. The use of these columns on standard analytical-scale chromatographs and also on automated preparative high-performance liquid chromatography systems, for the isolation of the title compounds, is described.

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