Abstract
Various adsorbents and solvent systems were compared with respect to the separation efficiency of four classes of isoflavones, malonylglucosides, acetylglucosides, glucosides and aglycones, in soybean cake by preparative column chromatography. The various fractions of isoflavones collected from column were monitored by HPLC. Soybean cake was found to contain malonylglucosides (803.70 μg/ml), acetylglucosides (85.57 μg/ml), glucosides (728.25 μg/ml) and aglycones (53.02 μg/ml). Both malonylglucosides and glucosides were separated using Diaion HP-20 as adsorbent and a solvent system of deionized water-ethanol with a ratio of 85:15 (v/v) and 73:27 (v/v), respectively. Whereas, both acetylglucosides and aglycones were separated using silica gel as adsorbent and a solvent system of n-hexane-isopropanol-ethanol (8:9:1, v/v/v). A total amount of 244.26, 21.25, 179.04 and 11.88 μg/mg were obtained for malonylglucosides, acetylglucosides, glucosides and aglycones, respectively.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
