Abstract
A carbon fiber microelectrode, surface of which ruthenium and glucose oxidase (GOx) were electrochemically codeposited, has been investigated. The Ru deposition onto the microelectrode increased current response to H 2O 2 oxidation, while decreased oxidation currents due to interfering substances, such as ascorbic acid, uric acid, p-acetamidophenol, l-cysteine and dopamine. The codeposition of Ru and GOx gave further suppression of the interfering signals with keeping the current response to H 2O 2. When amperometric glucose sensing was conducted by using the GOx and Ru modified microelectrode, an increase in GOx concentration in the deposition bath enlarged oxidation current of H 2O 2 generated from glucose oxidation by GOx. The presence of ascorbic acid in analyte gave no error in detection of glucose and errors caused by uric acid was +3% at the most for measuring 5 mM glucose, which is the normal physiological level in blood.
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