Abstract
Abstract In this study, the effect of the hydrophobicity of reduced graphene oxide (rGO) on the activity of candida rugose lipase (CRL) was investigated firstly. It was observed that the increasing hydrophobicity of rGO surface could increase CRL activity, while excessive hydrophobicity acted to depress its activity. Then, reduced graphene oxide beads (rGOB) had been fabricated through a facile self-assembly method. The morphology, specific surface area, and mechanical properties of the rGOB were investigated by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), and static mechanics properties test, respectively. The results revealed that the rGOB showed a three-dimensional micro-nano porous structure and surface hydrophobicity, which played an important role in improving their adsorption properties and catalytic activity of CRL. CRL was directly immobilized onto the beads by physical adsorption with the maximum adsorption capacity of 117.67 mg/g under the optimized condition. The immobilized lipase (rGOB@CRL) exhibited good temperature, thermal, and reuse stability in comparison to free CRL. In the glycerolysis reaction of olive oil, the conversion rate of triglycerides (TG) in free enzyme reaction system was 67.83%, while the conversion rate of TG in rGOB4@CRL reaction system could reach 74.87%, which was higher than that of the former. Moreover, rGOB@CRL was further used to adsorb emulsified oil due to the excellent oil-absorbing ability of rGOB, and then producing monoglyceride (MG) and diglyceride (DG) in situ by catalyzing the glycerolysis reaction. When the oil concentration was 4 mg/mL, the conversion rate of triglyceride (TG) could reach 89.70%.
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