Preparation of protein nanoparticle-coated poly(hydroxybutyrate) electrospun nanofiber based scaffold for biomedical applications

Abstract

In this study, soybean protein nanoparticles (SPN) modified poly(hydroxybutyrate) (PHB) electrospun nanofiber scaffold is fabricated by a facile two-step method combination of PHB electrospinning and SPN evaporation-induced self-assembly. The surface characteristics, structure and properties are investigated by SEM, EDS, XPS, FTIR, XRD, DMA, WCA, tensile test and water uptake capacity measurements. To characterize biodegradability and cytocompatibility, biodegradation tests are performed in simulated body fluid with and without lysozyme and cell cultures are carried out using NIH3T3 mouse fibroblast cells by in vitro studies. The as-obtained SPN-modified PHB nanofiber scaffold presents better cytocompatibility and is more suitable for biomedical applications.