Preparation of Poloxamer188-b-PCL and Study on in vitro Radioprotection Activity of Curcumin-Loaded Nanoparticles.

Xiaona Lin,Wenhui Li,Siyi Li,Meishuang Li,Mei Cong,Yuanbo Wang,Shasha Yu,Shengxi Chen,Yongli Shi

doi:10.3389/fchem.2020.00212

Abstract

A novel polymer of poloxamer188-b-PCL was synthesized via a ring-opening polymerization. Fourier transform infrared spectroscopy (FTIR), Raman, and 1H nuclear magnetic resonance (1H NMR) spectra were used to study the structures of obtained poloxamer188-b-PCL. The thermo-stability of poloxamer188 -b-PCL was carried out with a thermal gravimetric analyzer (TGA), and cytotoxicity was obtained using the CCK8 method. Cargo-free and curcumin (CUR)-loaded poloxamer188-b-PCL NPs were fabricated via the solvent evaporation method. The morphology, particle size distribution, and stability of cargo-free NPs were studied with a scanning electron microscope (SEM) and laser particle analyzer. The in vitro radioprotection activity of CUR-loaded NPs was performed. FTIR, Raman, and 1H NMR spectra confirmed that poloxamer188-b-PCL was obtained. TGA curves suggested poloxamer188-b-PCL had better thermo-stability than original poloxamer188. Cell tests suggested that the cargo-free NPs had no cytotoxicity. SEM image showed that the cargo-free NPs were spherical with a diameter of 100 nm. Free radical scavenging experiments proved that CUR-loaded NPs had better antioxidant activity than CUR solutions. CUR-loaded NPs could be detected in all tissues, including liver, kidneys and lung. In summary, this work demonstrated a feasibility of developing an injective formulation of CUR and provided a protection agent in caner radiotherapy.

Highlights

Curcumin (CUR), a natural chemical, is first extracted from the rhizomes of zingiberaceae or araceae plants (Zhao et al, 2015)
A novel block polymer of poloxamer188-b-PCL was synthesized via the ring-opening polymerization. 1H nuclear magnetic resonance (1H NMR), Raman, and Fourier transform infrared spectroscopy (FTIR) spectra confirmed that poloxamer188-b-PCL
The GPC curve showed that the Mw of poloxamer188-b-PCL polymers was 12,000 D

Summary

Introduction

Curcumin (CUR), a natural chemical, is first extracted from the rhizomes of zingiberaceae or araceae plants (Zhao et al, 2015). CUR is an infrequent diketone pigment which is distributed in plants. CUR is been one of the top-selling natural edible pigments. CUR has broad clinical application, i.e., antioxidant activity (Esatbeyoglu et al, 2015; Llano et al, 2019; Wang et al, 2019), anti-tumor (Ohtsu et al, 2002; Kunnumakkara et al, 2007; Yan et al, 2017), Radioprotection Activity of Curcumin-Loaded Nanoparticles anti-inflammatory (Aggarwal and Harikumar, 2009; Fadus et al, 2017; Hussain et al, 2017), and anti-hyperlipidemia (Huang et al, 2018; Panahi et al, 2018; Wang et al, 2018), and so on. Bioactivities of curcumin as an effective chemopreventive agent, chemo-/radio-sensitizer for tumor cells, and chemo-/radio-protector for normal organs, are of extraordinary research interests in the literature (Farhood et al, 2018)

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Conclusion