Abstract
This is a preliminary description of a relatively simple procedure devised for the isolation of purified and highly active preparations of adrenotropic hormone from whole hog pituitary glands. The extraction and fractionation procedures consist of the following steps: (1) Extraction and isolation of a fraction designated as crude prolactin by the procedure originally developed by Lyons1 and adopted by White, Bonsnes and Long2 as the first step for the subsequent preparation of crystalline prolactin. This “crude prolactin” contains adrenotropic hormone activity. (2) Solution of the crude prolactin preparation in 2% concentration at pH 9.0 and removal of precipitates which form by careful adjustment of pH to 8.0, 6.6 and 5.4. (3) Supernatant obtained in Step 2, after removal of pH 5.4 insoluble material (prolactin), is freed from traces of prolactin by the addition of 7 ml of saturated (NH4)2SO4 to each 100 ml of solution. The precipitate is separated and discarded. (4) Acetone is added to the supernatant t...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
