Preparation of Pectin Lyase by fermentation for customized extraction of paper pulp, viscose fiber, and nanofibrillated cellulose from hemp stalks

Abstract

The environmental and efficiently extracted cellulose technology with a high α-cellulose content from agricultural waste hemp stalks were treated by prepared Pectin Lyase (PL) and trace chemical additives. This extraction technique can obtain different α-cellulose content to meet the conditions for preparing pulp (PPs), viscose fibers (VFs) and nanofibrillated cellulose (CNFs). Specifically, fermentation process of PL (temperature, time, rotation rate and centrifugation rate) was evaluated on the reductive sugar concentration, fungus concentration and activity of hydrolase and lyase. Additionally, the morphological structure, chemical structure, crystallization structure, intermolecular action, and technical indicators of PPs, VFs and CNFs were characterized. The optimal results of fermenting PL were 10.3 U/mL of lyase activity and 36.1 U/mL of hydrolase activity. The technical indicators of PPs were 14.30 N·m/g of the horizontal ring pressure and 64 of the horizontal fold resistance times; The technical indicators of VFs were 2.13 cN/dtex of the intensity and 19.36% of the elongation at break; The length and width of CNFs were 326 nm and 3 nm, respectively.