Abstract
Pectin lyase was present in variable amounts in mitomycin C-induced lysates of 20 Erwinia chrysanthemi strains. In the KS612 strain, pectin lyase activity and the temperate bacteriophage (Erch 12) titre increased upon incubation of the cultures with mitomycin C; in these cultures pectate lyase activity remained at a basal level. Conversely, pectate lyase, but not pectin lyase, was induced with partially-esterified (citrus) pectin or polygalacturonate. The addition of rifampicin to mitomycin C-treated cultures prevented pectin lyase production. Pectin and pectate lyases were resolved by non-equilibrium pH gradient electrophoresis. Thus, pectin and pectate lyases of E. chrysanthemi strain KS612 appeared to be distinct molecular species, the production of which are differentially regulated. Pectin lyase production and cellular lysis were also observed in mitomycin C-treated cultures of E. carotooora subsp. alroseptica and E. carotooora subsp. carotovora. The level of pectin lyase generally was higher in E. carotooora subsp. carotovora than in other Erwinia spp. We conclude that pectin lyase production is a trait common to most soft-rot causing Erwinia spp.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
