Abstract
Palladium nanoparticle-bacterial cellulose (PdBC) hybrid nanofibers were synthesized by in-situ chemical reduction method. The obtained PdBC nanofibers were characterized by a series of analytical techniques. The results revealed that Pd nanoparticles were evenly dispersed on the surfaces of BC nanofibers. Then, the as-prepared PdBC nanofibers were mixed with laccase (Lac) and Nafion to obtain mixture suspension, which was further modified on electrode surface to construct novel biosensing platform. Finally, the prepared electrochemical biosensor was employed to detect dopamine. The analysis result was satisfactory, the sensor showed excellent electrocatalysis towards dopamine with high sensitivity (38.4 µA·mM−1), low detection limit (1.26 µM), and wide linear range (5–167 µM). Moreover, the biosensor also showed good repeatability, reproducibility, selectivity and stability and was successfully used in the detection of dopamine in human urine, thus providing a promising method for dopamine analysis in clinical application.
Highlights
Dopamine (DA) is an important neurotransmitter, which plays pivotal roles in the central nervous, cardiovascular and hormonal systems [1]
The Palladium nanoparticle-bacterial cellulose (PdBC) hybrid nanofibers were synthesized by in-situ chemical reduction method
The Pd nanoparticles were observed to be evenly dispersed on the surfaces of Bacteria cellulose (BC) nanofibers, which is beneficial for developing their electrocatalytic effect
Summary
Dopamine (DA) is an important neurotransmitter, which plays pivotal roles in the central nervous, cardiovascular and hormonal systems [1]. A variety of analytical techniques have been invented for the determination of DA, including optical spectroscopy [3], mass spectrometry [4], high performance liquid chromatography (HPLC) [5], and electrochemical methods [6,7]. Among these methods, electrochemical biosensors have attracted wide attention due to the great advantages of enzyme biosensors over conventional analytical techniques, like low price, high sensitivity/selectivity, rapid response and amenable miniaturization.
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