Abstract
To visually detect dopamine (DA) in real urine, the sensor responds to changes in wavelength and color was devised. A simple and green method to prepare gold nanoparticles (AuNPs) from Spirulina extract was presented. The weak protection of the alcoholic hydroxyl and carboxyl group of Spirulina polysaccharide/protein on S-AuNPs (AuNPs are prepared with Spirulina extract) were easily replaced by amino and phenolic hydroxyl groups in DA. As a result, the S-AuNPs began to agglomerate, and the color and plasmon resonance absorption peak of the S-AuNPs sol were changed at the same time. DA can be detected as low as 0.21 μM by UV–vis spectroscopy and observed by the naked eye (>5 μM). UV–vis analysis showed that the absorbance ratio (A696/A520) was linear to DA concentration (R2 = 0.99) in the range of 5–40 μM. This method has been applied to detecting DA in human urine samples, and the recovery rate ranged from 101.8% to 102.7%.
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