Preparation of oxaliplatin long-circulating liposomes and its effect on activity of human colorecal cancer SW480 cells

Abstract

Objective To investigate the preparation of oxaliplatin long-circulating liposomes and its effect on activity of human colorecal cancer SW480 cells. Methods The rverse-phase evaporation vesicles （REV） method was used to prepare the oxaliplatin long-clrculating liposomes. The particle size, electric potential, entrapment efficiency and face shape were analyzed, and the effects of liposomes on activity of cells were also analyzed. Results The particle size and electric potential were （ 151.56 ± 15.57） nm and （ - 23.68±2. 35） mV resepctively. Entrapment efficiency was （42. 96 ± 6. 45 ） %. At 2 h, the uptake of liposomes by cells was observed. At 2, 12, 24 h, immunofluoreseent intensity was 198, 443 and 642 respectively. After cells were treated with 2. 60 mmol/L blank liposomes, 28.00 mg/L free oxaliplatin and 2. 60 mmol/L liposomal oxaliplatin （ containing 28 mg/L oxaliplatin） , activity of cells was （ 84. 73 ± 1.73 ） %, （70. 72 ± 2. 66） % and （ 57. 69 ± 3.33 ） % respectively （ F = 104. 428, P 〈 0. 01 ）. Additionally, the number of cell colonies was reduced. Conclusion Oxaliplatin long-circulating liposomes were successfully prepared, and could enhance cytotoxic effects against colorecal cancer SW480 cells. Key words: Coloreeal carcinoma ; Oxaliplatin ; Liposomes