Abstract
In this work, we report a novel organic fluorescent nanocomposite for DNA detection. Through a reprecipitation method, π-conjugated oligomers 4,7-(9,9′-bis(6-adenine hexyl)fluorenyl)-2,1,3-benzothiadiazole (OFBT-A) incorporated Texas Red labeled oligonucleotides (named TR-P5) within their matrices to form OFBT-A/TR-P5 nanocomposites. Efficient fluorescence resonance energy transfer (FRET) was observed between OFBT-A and TR-P5. Introduction of DNA with different lengths has changed the aggregation structures of nanocomposites and thus altered the FRET efficiency. Based on the fluorescence signal responses of the OFBT-A/TR-P5 nanocomposites to different DNA, DNA elongation and cleavage processes were successfully monitored by simply observing the change of FRET efficiency of the nanocomposites solutions. This research provides a facile, fast and highly efficient manner for DNA analysis, which shows great potential in the study of DNA-related biological processes.
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