Abstract
Using allylamine monomer, a matrix of nanocones was fabricated by applying a low-temperature plasma plume without any catalysts and template. This nanocone acted as an adhesion layer immobilizing DNA probe for DNA hybridization assay. A simple conceptual model to describe the growth of the nanocones was also developed. The highest density of amino-labeled DNA probe was about 1.6pM∕cm2 confirmed by the dyed oligonucleotide, and each nanocone contained nearly 3×102 amine groups. This strategy provides a robust procedure to immobilize DNA, which is a very useful substrate for fabricating nanobiosensors.
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