Preparation of Nano Triblock Co-polymer for Desferoxiamine Delivery and Cross-Linked Copolymer for Iron Overload Disease

Iqbal J Al Asadi,Athir M Haddad,Isra A Q Falih

doi:10.4172/2471-9935.100020

Abstract

Some IPNs and copolymer were prepared at different ratios from bisacryl amide as crosslinking agent by Redox polymerization. These IPNs (polyacrylamide –poly ethylene imine); (polyacrylamide-poly electrolyte) and poly (acrylamide-acrylic acid) copolymer are examined by UV-VIS spectrophotometry method to determine their ability for linking with iron ions that are prepared with standard solution employed for this purpose. The results, indicate that the copolymer prepared from (AcAm:AcAc) with 20% (wt/wt) from bisacrylamide has the ability of removing the iron ions from the solution. The swelling ratio for the prepared IPNs and copolymers was studied as a function of time at different pH (1.2, 8.4) and distilled water; the obtained data show that the swelling ratio was increased with increasing the pH. The morphology of copolymers was performed by scanning electron microscopy (SEM) to show the pore size. A new triblock copolymer of (Polylactide-Pluronic127-Polylactide) is synthesized by ring opening polymerization using (DUB) Diazabicyclo-[5.4.0]-udec-7-ene as a catalyst. The triblock copolymer is characterized 1HNMR. Semi IPNs from triblock copolymer with acrylamide was prepared to use as a polymer drug delivery with Desferriox aminemethanesulphate (Iron overload chelating drug). The SEM for this semi IPN shows the nano structure due to the regular orientation of lactide fibers. Desferoxiamine release study as a function of time at pH 1.2 and 8.4 for 24 hours with semi IPNs coated with tetramethylourea and without coating was also carried out. The releasing studies show that the rate of releasing at GIF is larger than SGF.

Highlights

Hydrogels show reversible volume phase transitions depending on external stimuli factors such as temperature, solvent composition, ionic strength, pH, electric field, light, etc
Apart from the hydrogen bonding s and hydrophobic interactions, the coulombic attraction between oppositely charged monomer units, play a crucial role to determine their phase transitions that leads to a different chain configuration within the network structures.(Ogawa et al [4]) design a bio chemo mechanical poly hydrogel system consisting of NIPAM, acrylic acid (AcAc), and 1-vinylimidazole (VIM) units that undergo swelling and shrinking changes due to the electrostatic interaction between the opposite charges in the network. (Takeoka et al [5]) describe that poly hydrogels undergo a discontinuous phase transition depending on the interactions between its repeating units in the gels [6]
Acryl amide (AcAm), poly ethyleneimine (PEI) and bis acrylamide as crosslinker are dissolved in water (1%) sodium persulfate (Na2S2O8) is added as initiator and mixed well followed by addition of (50 μl) tetramethylethylenediamine as accelerator at the initiation step with stirring for 10 minutes at 35°C to complete the polymerization reaction

Summary

Introduction

Hydrogels show reversible volume phase transitions depending on external stimuli factors such as temperature, solvent composition, ionic strength, pH, electric field, light, etc. Acryl amide (AcAm) , Poly electrolyte and bis acrylamide as crosslinker are dissolved in water (1%) sodium persulfate (Na2S2O8) is added as initiator and mixed well followed by addition of (50 μl) tetramethylethylenediamine as accelerator at the initiation step with stirring for 10 minutes at 35°C to complete the polymerization reaction. Preparation of semi IPN loading with desferroxamine g Acrylamide, 1 g bis acryal amide, g PLA-P127-PLA tri block copolymer and 1 g Desferrioxamine methan sulphate (drug) are dissolved in 5 ml distilled water and mixed well sodium persulfate (Na2S2O8) and tetra methyl ethylene diamine are added with stirring at 35°C to complete the polymerisation reaction. The micrographs show presence of a large number of pores in different sizes On the other hand, Figure 4 shows presence of Nano structures which ranging from 35 nm to 95 nm

Result and Discussion

Findings

C O CH2 CH2 fg