Abstract
To promote the application of microbially induced carbonate precipitation (MICP) technology for coal dust suppression, two urease-producing bacteria (SZS1-3 and SZS1-5) in a coal mine soil were screened. 16 s rDNA sequencing of these bacteria indicated sequences similar to those of Acinetobacter guillouiae CIP 63.46 and Staphylococcus caprae ATCC 35538, respectively. Strains SZS1-3 and SZS1-5 had their largest urease activity at the initial urea concentration of 0.5 mol/L, under slightly alkaline (pH = 9) and neutral (pH = 7) initial pH levels, respectively. Their final mineralized products were vaterite-type and calcite-type calcium carbonate. Although dust suppression results indicated that both bacteria can effectively bind coal dust particles, consolidated coal dust treated with SZS1-5 had a stronger resistance to wind and rain. The screening, mineralization and dust suppression potential analysis of these two urease-producing bacteria can provide reference for the application of MICP technology in coal dust suppression.
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