Abstract
One microgram to 1 mg of organic acid was completely converted to its methyl derivative when it was dissolved in methanol and treated with an excess of ethereal diazomethane. Single products were obtained from α-ketoglutaric acid, oxaloacetic acid, pyruvic acid, glyoxylic acid, or hydroxypyruvic acid on reaction with thionyl chloride – methanol. The separation of 24 organic acids by gas–liquid chromatography on a Reoplex 400 column is presented.
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