Abstract
Summary An enzymatic method is described for the preparation of viable and stable protoplasts from cell suspension cultures of Lolium endosperm. A new purification method was developed using a conjugate of Sepharose 4B and the mouse myeloma protein J539 (an anti-galactan agglutinin). The particulate contaminants bound to Sepharose-J539 forming large aggregates which could be separated from the protoplast suspension by simple filtration.
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