Preparation of Inhalable Salbutamol Proliposome Using Different Core Carriers and Investigate the Impact of Simulated Lung Fluid Use in Evaluation

Abstract

Objective: Pro-liposomes are free flowing precursor of liposomes. Liposome low stability can overcome by preparation of Pro-liposomes as high stability precursor of liposome produced upon hydration. Salbutamol sulphate is bronchodilator widely used for the treatment of asthma. Because of its high water solubility its penetration through lipid barriers is low. The preparation of water soluble drugs as liposomes improves their solubility across biological membranes. Method: Microparticulate sucrose, mannitol, trehalose, and lactose separately as core carriers were prepared using mini spray dryer. Ethanolic lipid (soya phosphatidylcholine) solution having salbutamol sulphate and micro particulate core carriers at 1:5 w/w phospholipid to carrier ratio was spray dry to find proliposomes. The prepared liposomes were evaluated for particle size distribution, polydispersity, Z.P., entrapment capacity, and morphology. This investigation was undertaken to compare the evaluation results of the produced liposomes that hydrated by simulated lung fluids that mimic human interstitial lung fluid and deionized water. Results: Good proliposomes were obtained with different properties using spray drying. The results show that carrier type has significant effect on the properties of prepared liposome. In addition, there are obvious differences in results between liposomes generated in simulated lung fluid and deionized water. Overall, simulated lung fluid had significant effect on liposomes size, polydispersity, zetapotential, and entrapment efficiency but had minimal effect of liposome morphology. Conclusion: It can be concluded that use of simulated lung fluid is preferable than deionized water in evaluation of prepared liposomes.