Preparation of Immobilized Lipase on Silica Clay as a Potential Biocatalyst on Synthesis of Biodiesel

Ting Zou,Qiao-E Wang,Hai-Ming Cheng,You-Dan Duan

doi:10.3390/catal10111266

Ting Zou, Qiao-E Wang + Show 2 more

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https://doi.org/10.3390/catal10111266

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Abstract

Biodiesel offers an important alternative to fossil fuel. In this work, Eversa Transform 2.0 lipase was immobilized onto 3-aminopropyltriethoxysilane (APTES) modified silica clay (SC) by glutaraldehyde. The characteristics of the functionalized supports and the immobilized lipase were investigated by FTIR, TEM, BET, and XRD. The results show that the optimal conditions of lipase immobilization are as follows: 2% glutaraldehyde concentration, 15 mg/mL lipase concentration and incubating at 25 °C for 60 min. The immobilized lipase showed a high tolerance to temperature and pH variation in comparison to the free lipase. The immobilized lipase on SC was applied as a biocatalyst for the synthesis of biodiesel from methanol and canola oil. A biodiesel yield of 86% was obtained at a temperature of 45 °C via a three-step methanol addition. A conversion yield of 67% was maintained after reusing the immobilized lipase for five cycles. This work provides a strategy for the preparation of an efficient biocatalyst for the synthesis of biodiesel.

Highlights

Biodiesel is a clean alternative fuel which can be produced by renewable natural resources
The results show that Lip–Glu–A-Silica clay (SC) is more tolerant than the free lipase at a relatively high temperature
Silica clay was modified by APTES and crosslinked with glutaraldehyde for the immobilization of lipase (Lip–Glu–A-SC)

Summary

Introduction

Biodiesel is a clean alternative fuel which can be produced by renewable natural resources (oils and fats). The transesterification of triglyceride is generally carried out by a catalyst, which can be acid, alkali, or enzyme catalysts [8]. Acid catalysts, such as sulfuric acid, hydrochloride, and phosphoric acid, are applied in biodiesel production due to their efficient catalytic performance. Enzyme-catalyzed transesterification has led to the development of recyclable and environmentally friendly catalysts. Such a catalyst has been considered to be the most promising prospective technology for biodiesel production [12]

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