Abstract
Abstract A method is proposed for the germination of fungal-free seeds of Magnolia grandiflora as a source of tissue for in vitro experiments. A combination sequence of a 16 hour water-soak at 25°C, hot water (52°C) for 1 hour, and then surface sterilization for 1 hour in 1:1000 aqueous mercuric chloride was the best treatment. Dips in 2.5% chlorox, 95% ethyl alcohol and concentrated sulfuric acid soak for 20 minutes were unsatisfactory. If seeds are selected carefully and treated as described, 60 to 90% of the seeds will germinate aseptically within 30 days.
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