Abstract
Abstract Techniques of experimental neuroanatomy are those that enable one to characterize the position of neurones in the microcircuits of the nervous system on the basis of their morphology, chemistry, and connections. The essence of the techniques are that the morphology, chemistry, and output region of neurones are identified first at the light microscopic level and then the chemistry, origin, and pattern of afferent synaptic input are identified at the electron microscopic level. Information of this nature is a pre-requisite to the characterization of the microcircuitry or neuronal networks of a region and provides a framework within which functional data can be interpreted. For the analysis of individual neuronal elements or populations of neurones in the microcircuitry of an area it is necessary to examine the tissue at the light microscopic level. For the analysis of synaptic interactions of individual neurones or populations of neurones, it is necessary to examine the tissue at the ultrastructural level with an electron microscope. The object of this chapter is to introduce to the reader the basic techniques used in the preparation of tissue from neuroanatomical experiments for light and electron microscopy.
Published Version
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