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Abstract
In the present work, cell-specific peptides were immobilized on chitosan and PLA scaffolds. The amount of immobilization, determined by HPLC, was confirmed to be in the effective range. Results from cultures of human fetal skin fibroblasts, rat osteosarcoma, and periodontal fibroblasts demonstrated that the immobilization of cell-specific peptides could effectively enhance the attachment and mineralization of osteoblastic cells on scaffolds. The results indicate that, with immobilization of osteoblast-specific peptides, scaffolds with specificity to osteoblastic cells can be obtained which are believed to be of practical use for generation of bone-like tissues.
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