Preparation of bovine serum albumin‐imprinted calcium polyacrylate/alginate hybrid microspheres via Ca2+ crosslinking

Abstract

AbstractMacromolecularly imprinted calcium polyacrylate/alginate hybrid polymer microspheres with the imprinting of bovine serum albumin were prepared with sodium alginate and sodium polyacrylate by using CaCl2 as a gelling agent in inverse‐phase suspension. Infrared spectrum and conductance titration demonstrated that hybrid components were produced in these microspheres. Rebinding isotherms of the microspheres proved a greater bovine serum albumin affinity for imprinted microspheres relative to nonimprinted ones. Selectivity tests indicated that calcium polyacrylate/alginate hybrid polymer microspheres exhibited good recognition properties for the template protein with the separation factor over 5.30 comparing to the competitive proteins with a similar Mw. The influence of cross‐linker CaCl2 concentration on the imprinting efficiency (IE) and rebinding capacity was researched. It was found that the cross‐linking density and imprinting efficiency of the hybrid hydrogel could be improved simultaneously. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009