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Abstract
AbstractMacromolecularly imprinted calcium phosphate/polyacrylate/alginate (CP/PA/A) multi-hybrid polymer microspheres with the surface and embedded imprinting of bovine serum albumin were prepared with sodium alginate (SA), (NH4)2HPO4 and sodium polyacrylate (SPA) by using CaCl2 as gelling agent in inverse-phase suspension. Infrared spectrum analysis demonstrated that multihybrid components were produced in the microspheres. Rebinding dynamic of both imprinted microspheres proved a higher BSA rebinding relative to those nonimprinted ones. The specific surface area of CP/PA/A microspheres increased when compared with non-imprinted ones. The specific rebinding peak and imprinting efficiency cross point have appeared in rebinding dynamic curves, which showed remarkable difference in rebinding behaviours between surface and embedding imprinted microspheres.
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