Abstract
A novel functional electrode was obtained by implanting NH2 + into ITO film (NH2/ITO) for the first time. The NH2/ITO surface showed a better affinity to gold nanoparticles than bare ITO. Gold nanoparticles were deposited on the surface of NH2/ITO electrode (Au/NH2/ITO). The Au/NH2/ITO and NH2/ITO electrodes were used to observe the electrochemical behavior of Hemoglobin (Hb) immobilized on the electrodes surfaces. The peak current value of Hb immobilized on NH2/ITO increased compared with on bare ITO while peak current value of Hb immobilized on Au/NH2/ITO increased compared with on Au/ITO. Linkage between the ‐NH2 implanted into the ITO film and the ‐COOH of Hb was thought to be the reason for the increase of active Hb coverage on NH2/ITO compared with bare ITO. Increase of active Hb coverage on Au/NH2/ITO compare with Au/ITO was attributed to the different amount of gold nanoparticles deposited. Results showed the novel NH2/ITO and Au/NH2/ITO electrodes exhibited good stability, reproducibility besides selectivity and sensitivity. The electrode process of Hb immobilized on Au/NH2/ITO was quasi‐reversible with adsorption. The electrode reaction rate constant ks and other related constants were determined. X‐ray photoelectron spectroscopy (XPS), field‐emission scanning electron microscopy (FE‐SEM), and impedance spectra were used to characterize the different surfaces.
Published Version
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