Abstract
A novel electrode was prepared by implanting NH2 + into an ITO film (NH2/ITO). Gold nanoparticles were deposited on the surface of NH2/ITO electrode. The NH2/ITO and Au/NH2/ITO electrodes were used to determine hemoglobin (Hb) immobilized on the electrodes surfaces. The relationship of the reductive peak current value of Hb among different electrodes was: Hb/ITO:Hb/Au/ITO:Hb/NH2/ITO:Hb/Au/NH2/ITO=1:1.5:2:4. The linkage between the –NH2 implanted into ITO film and the –COOH of Hb was recognized to be the reason for the increase of active Hb coverage on NH2/ITO electrode compared with the ITO electrode. Increase of active Hb coverage on Au/NH2/ITO compared with Au/ITO was attributed to the different amount of gold nanoparticles deposited. The determination of Hb at an Au/NH2/ITO electrode was optimized. Calibration curve was obtained over the range of 1.0 × 10−8 – 1.0 × 10−6 mol · L−1 with a detection limit of 1.0 × 10−8 mol · L−1. Results showed that the novel NH2/ITO and Au/NH2/ITO electrodes exhibited good stability, reproducibility besides better electrochemical performance.
Published Version
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